State farm intership explanation. What I learned and did day to day.  

Over time, large organizations accumulate hundreds of different subscription services that can be hard to keep track of. If an employee wants a tool in order to accomplish a certain task, it can take a while for the IT department to identify whether an existing subscription to a tool can satisfy the need.

To satisfy this need, we designed a custom AI model that can answer these questions. We started off with a Claude Opus 4.6 model and customized it for this particular job using prompt engineering, fine-tuning and thorough testing. This model allows employees to efficiently find out whether Fulton Bank has an existing subscription that matches their use case, and if multiple subscriptions could potentially work, the model gives advice on how to choose between the different possible tools.

Secondary succession refers to the gradual, predictable changes a habitat undergoes in structure, plant composition, and resource availability over time, yet few studies quantify these changes across multiple decades. The expansion of Raystown Lake in central Pennsylvania in 1973 removed human activity from much of the area, transforming a largely agricultural landscape into one capable of undergoing natural succession. Since then, this process has reshaped the region’s ecology. We assessed the impact these changes have had on small mammal communities in the area by replicating a survey first conducted in the summer of 1973 and repeated in the spring of 1998. During the summer of 2025, we re-established sixteen historical trapping grids within meadow and forested habitats near Raystown Lake. Each grid consisted of twenty-five Sherman live traps spaced six meters apart and sampled for five consecutive nights.

Across all trapping efforts, we recorded 123 captures representing 53 unique individuals and three species: Peromyscus leucopus, Peromyscus maniculatus, and Tamias striatus. Community composition differed markedly from historical surveys, with species diversity highest during the 1970s when agricultural fields dominated the landscape and lowest in 2025, when captures were overwhelmingly dominated by P. leucopus. Trap success was positively associated with pinecone abundance, coarse woody debris, bare ground, and canopy cover, while herbaceous groundcover and humidity negatively influenced capture rates. These findings suggest that long-term land-use change and forest succession have reduced small mammal diversity while favoring forest-adapted generalist species.

Self-efficacy, simply defined, is one’s belief about what they can do (Bandura, 1977). Rooted in social cognitive theory, this concept highlights the interactions among three sets of influences: personal, behavioral, and environmental (Schunk & DiBenedetto, 2014). Self-efficacy is tied to one’s belief in the ability to perform a specific task or behavior. These beliefs then drive related actions that support the attainment of a goal, which is situated within the supports and barriers of a specific setting. Social cognitive theory is the foundation for social cognitive career theory (SCCT), which similarly explains the connection between cognitive-person variables (e.g., thoughts and behaviors) and environmental factors and how they relate to an individual's academic and career interests, goals, and motivations (Lent et al., 2005).

 According to SCCT, self-efficacy is positively associated with one’s interests, outcome expectations, and available support (Lent et al., 2003; 2005; 2008). In addition, it has been found to be associated with academic and career choice objectives, such as the selection of and persistence in an academic major (Lent et al., 2003). Similarly, academic self-efficacy was associated with academic performance and personal adjustment to college in first year students (Chemers et al., 2001). In this study, academic self-efficacy was tied to academic expectations and performance, even when controlling for high school GPA. In addition, academic self-efficacy was indirectly tied to students’ perceptions of their abilities to handle academic challenges. Consequently, students with higher academic self-efficacy likely exhibit beliefs and behaviors that support their adaptation to and success in college. Understanding the role of academic self-efficacy beyond the first semester of college, however, may be important for understanding and supporting student success throughout the college experience.  

Although academic self-efficacy has been identified as a factor associated with academic and career goals and outcomes (Lent et al., 2003; 2005) and academic adjustment (Chemers et al., 2001), the majority of research on academic self-efficacy examines this variable at one point in time. Those that have examined it longitudinally were limited in scope of time frame, such as one academic year (Chemers et al., 2001) or within one semester (Lent et al., 2009). Additionally, much of the research has focused on first-year students, who are juggling the transition to college with potential uncertainty about their current and future academic and career goals. Knowing that academic self-efficacy is tied to one’s beliefs, behaviors, and the interaction of these with environmental factors, it would be beneficial to understand how these factors may change over the course of one’s undergraduate education.

Students’ experiences shape the development of new or different goals or beliefs. Through their college career, it is expected that they will need to acclimate to challenges and make adjustments as needed to attain their goals.  Consequently, it is expected that dimensions of self-efficacy will likely change over time, but increase in their senior year. The aim of our exploratory study is to examine academic self-efficacy across time, from first to fourth year, during their undergraduate education.

Correlation Between Physical Activity Levels and Perceived Stress Among International College Students

   Rylea N. Lynch1, Tiago V. Barreira2, Kamala Ramadoss2, Joon Young Kim2, Andrew R. Heckel1,2
1Juniata College, Huntingdon, Pennsylvania, 2Syracuse University, Syracuse, NY

 

INTRODUCTION: International college students face challenging environmental and psychological challenges, which can lead to an elevated stress perception. Studies have shown that approximately 70% of international college students experience high levels of stress. Engaging in physical activity has been shown to help improve well-being and reduce stress, with all physical activity levels having shown benefit for reducing perceptions of stress. However, it is unknown whether light (LPA) and moderate to vigorous physical activity (MVPA) are differentially related to stress levels in international college students. PURPOSE: To compare the strengths of relationship between LPA and MVPA on perceived stress scores in international college students. METHODS: A total of 47 international college students were included in this study (age: 25.4 ± 4.2 years, females: 18). To quantify perceptions of stress, we utilized the perceived stress scale (PSS-10). Accelerometry was used to assess physical activity levels, light and moderate to vigorous activity levels were defined using previously established cutoff points. Using Pearson correlation coefficient, the relationship between PSS-10 scores and physical activity levels were examined. The Pearson correlation coefficients were then compared using Fisher’s r-z transformation. RESULTS: Pearson correlation coefficients revealed that MVPA (r = -090) and LPA (r = 0.004) were weakly and insignificantly associated with PSS-10 scores in our cohort of international college students (p > 0.05). According to Fisher’s r-to-z transformation, there were no significant differences in the strength of the relationship for MVPA and LPA with PSS-10 scores (p > 0.05). CONCLUSION: Overall, no significant difference in benefit in PSS-10 was found between light physical activity and moderate to vigorous physical activity. Light and moderate to vigorous physical activity have similar effect on stress in international college students. 
   The authors declare no conflicts of interest.

Open Quartermaster is an open source inventory management system, and in order to simplify and streamline it's use, we are building a hardware scanner that will be able to add and remove items from an OQM inventory

Using a convenience sample, participants will be participating in an exploratory study and will be asked to complete a survey assessing their sleep habits, athletic performance and academic performance in order to help gain knowledge of sleeping behavior on campus. Some of the variables and measurements we will be looking at are Demographics, Sleep Habits, Quality of sleep with the PSQI and Self-Reported, Are you an athlete and what sport do you play? Perceived Performance which will be Self-Reported. We are using a convenience sample within a survey to see if Unhealthy sleep patterns in collegiate athletes may result in poor performance in athletics and academics.

This study investigates the effectiveness, affordances, limitations, and family perceptions of conversational AI for home literacy learning vs. human. We developed a large language model (LLM)-powered conversational AI system, named Vovo, to teach children vocabulary and co-construct stories using structured literacy pedagogy. The system was tested in home environments over six weeks with 10 families and their children aged 3–7 (M =5.4). Across 150 learning sessions, Vovo delivered structured literacy instruction as effectively as parents, though children achieved higher learning outcomes when learning with parents. Video analysis revealed Vovo's advantages in pedagogical consistency, language modeling, and verbal socioemotional support, while facing challenges in speech recognition, instructional persistence, nonverbal social cues, and phoneme instruction. Parents perceived Vovo as intelligent, useful, and trustworthy, while expecting a multimodal design to improve engagement. Children perceived Vovo smart and fun but still preferred learning with parents due to emotional bonding. As one of the first studies to embed structured literacy pedagogy into home-based conversational AI system, this research contributes empirical insights into the evolving role of AI in home literacy environments. It also underscores the socially responsive AI design in early education and calls for future design that support parent-child-AI triadic interactions to optimize AI in home literacy learning.

This study examines the extent to which Juniata College students understand the CSI effect and forensic DNA evidence, and whether exposure to forensic television influences those perceptions. Using a pre- and post-survey design, we compared students enrolled in a CSI-effect-focused course with students in two psychology courses to assess changes in knowledge over time. Prior research suggests that popular forensic dramas such as CSI: Crime Scene Investigation and its spinoffs have shaped public expectations about the speed, reliability, and accuracy of DNA evidence in criminal investigations. Although some studies indicate that the CSI effect does not substantially bias jury verdicts, research shows that viewers often place greater trust in DNA evidence despite limited scientific understanding. Drawing on scholarship related to media influence, juror decision-making, and forensic practice, this study explores whether similar patterns exist among college students. The findings may benefit the Criminal Justice program at Juniata College by identifying gaps in forensic knowledge, informing curriculum development, and strengthening students’ critical understanding of DNA evidence, investigative limitations, and courtroom realities.

Amphibians such as salamanders, newts, and frogs are sensitive populations that serve as indicator species, providing valuable information regarding ecological health. Habitat destruction, invasive species, and environmental contaminants may alter amphibian populations, behaviors, and dispersal. Historically, amphibian populations are surveyed, measured, and monitored through acoustics and observations (calls, egg mass surveys, visual observation), catch-and-release, funnel traps, and pitfall traps. Observational techniques are labor intensive and are prone to data inaccuracies. Catch-and-release techniques, even when executed properly, can cause stress, degrade protective mucous layers, damage skin, and expose them to toxins that are easily absorbed. Trapping methods can result in mortality and require considerable efforts from researchers. The Adapted-Hunt Drift Fence Technique (AHDriFT) is a passive, low-cost, low-effort, long-term monitoring system used to study herpetofauna. Through the utilization of game camera technology mounted within an inverted bucket at the end of a drift fence, amphibians and reptiles are guided through the bucket and photographed. This method is non-invasive, minimizes stress, and reduces harm to the subjects, consequently reducing individual mortality. To study the abundance, species richness, and life stages of amphibians in Huntingdon, Pennsylvania, we launched two AHDriFT arrays at a constructed vernal pool and a natural vernal pool in the spring of 2026.

Imagine n drivers arriving at a row of n parking spaces and each trying to park in a preferred spot — sometimes they must move right to the next free space. If all the cars can park without backing up, the list of those first preferences is a combinatorial object called a parking function. In this project I give a hands-on procedure that turns a (non-decreasing) parking function into an up-and-right lattice path (a Dyck path), and then into a rooted binary tree. The rules are concrete and visual, so the whole process can be drawn step by step. The project describes which preference patterns produce general versus binary trees, and explores a few natural statistics that are preserved or transformed under the map. This makes an accessible, visually engaging story connecting three classical families of combinatorial objects. 

This poster presentation features student research from the Gender and Conflict course, exploring how gender shapes the causes, experiences, and impacts of conflicts and wars worldwide. Through diverse case studies, students examine issues such as gender-based violence and the intersection of gender with social and political power. The projects highlight the importance of gender-sensitive perspectives in understanding conflict and building more inclusive pathways to peace.

Current experimentation in Juniata College’s Cognitive Affective Motor Lab extends previous work in which the effects of stress on learning is evaluated. Prior investigations utilizing the presence (or absence) of visually and behaviorally professional experimenters, awareness of performance evaluation, access to knowledge of standards of good performance, yielded an anxiety manipulation which was weaker than anticipated and/or desired. Methods were augmented with the additional inclusion of a brief (10ms) exposure to 110dB white noise when participants perform poorly on the experimental task: a motor sequence learning task. The present analysis compares subjective anxiety reports after initial exposure to the stressor suite from previous iterations to the experiment to that from the current, ongoing data collection process to evaluate whether the revised procedures more efficiently elicits subjective reports of state anxiety. Assessment was performed using the State-Trait Anxiety Inventory (STAI), as well as a simple Visual Analog Scale (VAS) targeting anxiety. It is hypothesized that the updated method produces higher levels of self-reported anxiety than the original, indicating a stronger, more effective manipulation. Results will be presented and discussed.

Vernal pools are isolated, ephemeral wetlands that form naturally based on local geology and soils, or result from historical human activity, like mining. Test pits dug for mining operations formed many isolated ponds in Central Pennsylvania that are now occupied by a variety of organisms, including macroinvertebrates and pond-breeding amphibians. Natural and constructed vernal ponds supply food, cover, and water for local wildlife, contributing to biodiversity. Recognizing the ecological value of these habitats, the U.S. Army Corps of Engineers partnered with Blue Acres, LLC., and constructed six vernal pools across the Raystown Lake Project area in 2022 and 2023. From 2024-2026, we monitored five of the constructed ponds using visual encounter surveys, camera traps, dip-netting surveys, and minnow trapping to determine the presence and absence of wildlife. Across two years of sampling, we found that three of the constructed pools had diversity levels comparable to natural pools of the region. We offer additional recommendations for constructing vernal pools and monitoring. 

This study aims to quantify microplastic accumulation in White Suckers (Catostomus Commersonii), Brown Trout(Salmo trutta), and Brook Trout (Salvelinus fontinalis) collected from local streams representing varying degrees of water quality, ranging from remote streams to heavily impacted streams. Microplastics were examined within three different organ systems, the GI tract, brains, and gills, to identify variation in retention and accumulation between among the different organs. Age, weight, and length of the fish were recorded to assess any potential relationships between microplastic count, age, and morphological measures. Since the main pathway to microplastic accumulation is through ingestion from aquatic food sources, this research can allow for interpretations of microplastic exposure and distribution in local waterways which are applicable for smaller communities that rely heavily on local rivers to provide a large supply of their drinking water. This can provide an insight for the community on about how many microplastics they are being exposed to in their local area. Microplastics continue to emerge as a concerning pollutant in freshwater systems, and this study allows for an insight into their movement, potential impacts on fish health, and the implications for human populations in rural communities. 

Amphibians are among the most threatened vertebrates, with climate change, habitat loss, and disease driving many declines and extinctions. Sensitivity to environmental change and pathogens is heightened by amphibians' permeable, glandular skin, which facilitates direct cutaneous respiration and water absorption but offers little barrier against waterborne toxins, pollutants, or pathogens like Batrachochytrium dendrobatidis, making them exceptionally reliable indicators of ecosystem health. Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal) are two chytrid fungal pathogens causing amphibian declines across the globe. In North America, Bsal has not been detected in North America as of 2026, while Bd has been detected as early as 1928. The Student Network for Amphibian Pathogen Surveillance (SNAPS) is a collaborative network that aims to provide experiential learning and teamwork to students, while providing students with the resources needed to surveil for emergent pathogens like Bsal and Bd. 12 students across three semesters swabbed 61 individual amphibians across 6 sites. Overall prevalence of Bd across the sites was 9.8%, with one site having a prevalence rate of 16% which may be due to a variety of factors, including temperature and human activity.  

Anguilla rostrata, or the American eel, is a significantly understudied species that can provide much insight into the contaminants present in various ecosystems. Analyzing Anguilla rostrata as a marker for microplastics can provide important data for both organisms in Pennsylvania waterways and various other ecosystems. Since Anguilla rostrata travel vast distances to spawn in the Saragossa Sea but live most of their lives in freshwater ecosystems in northeastern America, they are exposed to pollutants on a much larger scale than most aquatic organisms found in Pennsylvania. Our research developed a three step approach to analysing microplastics in this species, consisting of organ removal via dissection, KOH digestions/vacuum filtration, and microplastics identification/analysis using light microscopy. The findings of this work demonstrated the presence of microplastics in tested samples, including muscle tissue, gills, brain, and eyes. The most shocking of our findings was the incredibly high concentration of microplastics in the brain compared to the other organs sampled. Such findings imply that microplastics are not simply found in organs exposed to the environment, such as the eyes or gills, but rather have worked their way into internal organs such as the brain despite factors like the blood brain barrier. Microplastics are thus circulating through organ systems that have no direct contact with these environmental pollutants. As this project continues additional organs such as the liver, GI tract, pancreas, and stomach are being dissected and analyzed for microplastics to advance our understanding of how microplastics translocate through the body on a broader scale. Additional genetic sequencing is being performed on select GI tract samples to identify possible relationships between the gut microbiome composition and the presence of microplastics. 

College life represents a critical developmental period characterized by new academic, social, and personal demands. While this phase offers opportunities for growth, it is also marked by heightened stress and challenges to overall well-being. Recent research has revealed a concerning trend, a steady decline in college students’ mental health over the past decade (Eisenberg et al., 2014; 2017; 2025). Rising levels of stress, anxiety, and depression have contributed to a growing number of students experiencing psychological distress and an increased demand for on-campus counseling services (Kitzrow, 2003). These mental health challenges not only influence students’ emotional well-being but also impact critical outcomes such as academic performance, retention, and graduation rates (Kitzrow, 2003). 

Because of these concerns, it has become increasingly important to explore how stress, coping mechanisms, and psychological well-being interact within the college population. Understanding these relationships can provide valuable insight into developing targeted interventions and support systems that not only reduce distress but also promote resilience, personal growth, and academic success.

Stress

Stress exerts both physiological and psychological effects on students, influencing not only their academic performance but also their overall well-being (Baghurst & Kelley, 2014). Research shows that stress can stem from a variety of sources, both academic and non-academic. These include the constant pressure to achieve high grades, uncertainty about post-graduation plans, financial strain, poor quality of sleep, declining physical health, and even dissatisfaction with body image (Beiter et al., 2014).

High levels of stress have been linked to psychological distress and lower self-esteem (Saleh et al., 2017), and when left unmanaged, chronic stress may contribute to more serious mental health issues such as anxiety and depression (Beiter et al., 2014). Ultimately, the degree to which stress impacts college students depends not only on the intensity of these external pressures, but also on how individuals perceive and respond to them through their coping mechanisms and resilience strategies (Graves et al., 2021). 

Coping

Coping mechanisms play a vital role in managing stress and supporting overall psychological well-being, particularly among college students who often face a wide range of academic and personal challenges (Freire et al., 2016). Scholars generally categorize coping strategies into three main types: problem-focused, emotion-focused, and avoidant coping (Litman, 2005). Students who engage in problem-focused coping – actively addressing the source of stress through planning, goal setting, or seeking solutions – tend to report higher levels of optimism and self-esteem (Aspinwall & Taylor, 1992). In contrast, students who rely heavily on avoidant coping mechanisms, such as denial, withdrawal, or disengagement, often experience heightened levels of stress, decreased motivation, and poorer overall well-being (Bowman, 2010). These findings suggest that cultivating proactive and adaptive coping strategies may be key to fostering long-term mental health and success in academic environments. 

Wellbeing

Psychological well-being reflects an individual’s ability to realize their potential and engage in life with a sense of purpose and fulfillment (Ryff, 1989).            Ryff’s (1989) influential model of psychological well-being provides a comprehensive framework for understanding positive mental health along six key dimensions: self-acceptance, positive relations with others, autonomy, environmental mastery, purpose in life, and personal growth. Each of these dimensions contributes to a sense of well-being. Among college students, these elements often manifest as growing self-confidence, the formation of meaningful peer relationships, the ability to make independent decisions, and the pursuit of long-term goals that give life direction and meaning. 

Research has demonstrated that higher levels of psychological well-being are strongly associated with adaptive coping styles – particularly the use of problem-focused coping strategies that encourage students to confront and manage stressors directly (Freire et al., 2016). These findings highlight the importance of fostering Ryff’s dimensions of psychological well-being through healthy coping strategies that promote resilience, autonomy, and a sense of personal growth in college students. 

Present Study

This cross-sectional study, using self-report quantitative measures, aims to gather a deeper understanding of the relationship between perceived stress, psychological well-being, and coping. These measures were collected pre-COVID in the fall of 2019 and then again, the fall of 2025. This study hypothesizes that within each time period lower psychological well-being is associated with higher stress and using less adaptive coping mechanisms. Based on the trends in mental health, this study also hypothesizes that from the fall of 2019 to the fall of 2025 stress levels would be greater, less adaptive coping mechanisms would be in use, and overall psychological well-being would be lower.

Method

Participants

From the fall semester data collection in 2019 there were 200 participants. These participants were all undergraduate students at Juniata College. There are 109 undergraduate participants for this fall semester from Juniata College. 

Measures

Perceived Stress Scale (Cohen et al., 1983). This scale consists of 10 items rated on a 5-point Likert scale ranging from 0 (never) to 4 (very often). Higher scores indicate greater perceived stress levels. 

COPE Inventory (Carver et al., 1989). This measures the cognitive and behavioral efforts individuals use to manage stress. It includes distinct dimensions: problem-focused, emotion-focused, and avoidant. Items are rated on a 4-point Likert scale ranging from 1 (I usually don’t do this at all) to 4 (I usually do this a lot), with higher scores indicating greater use of that coping strategy.

Ryff Psychological Wellbeing Questionnaire (Ryff, 1989). This measures six dimensions of psychological wellbeing: self-acceptance, positive relations with others, autonomy, environmental mastery, purpose in life, and personal growth. Each dimension is represented by seven items rated on a 6-point Likert scale (1=strongly disagree to 6=strongly agree), with higher scores indicating greater levels of psychological wellbeing.

Data Analysis Plan

Bivariate correlations will examine both within-and across year relationships between perceived stress, coping strategies, and psychological well-being. An ANOVA will be used to identify differences in these variables across time (2019-2025). Together, these analyses will assess cross-sectional trends in college students’ stress, coping, and psychological well-being. 

Discussion

This study is limited due to unequal sample sizes and self-report measures may reduce generalizability. Future research should explore additional factors, such as social support or other factors that may shape and influence students’ stress, coping, and wellbeing.

The Association Between Systemic Inflammation and Balance Function in the U.S. Adult Population
   Shubh Sethia, Georgios A. Stylianides, Andrew R. Heckel
Juniata College, Huntingdon, PA

In the United States (US), the leading cause of injury related emergency room visits are fall related injuries. Balance function has been shown to be a good indicator of fall risk, with individuals that performed poorly on the Modified Romberg Test (MRT) of Standing Balance on Firm and Compliant Support Surfaces having a greater than 3 fold increase in the odds of falling. Higher levels of systemic inflammation are related to worsened function of the systems that govern balance function. However, no study has investigated the relationship between systemic inflammation and balance function.   PURPOSE: To investigate whether C-reactive protein (CRP), a biomarker of systemic inflammation, is significantly associated with balance function in the US adult population. METHODS: A total of 2,040 participants from the 2021-2023 National Health and Nutrition Examination Survey were included in this study (44.3 ± 13.2 years, female: 55.2 %). Systemic inflammation was defined as serum CRP levels measured using a two-reagent immunoturbidimetric system. Balance function was defined via the total number of trials passed on the MRT. The following covariates were included due to their potential effects on the relationship between systemic inflammation and balance function: age, gender, race/ethnicity, education level, socioeconomic status (SES), alcohol consumption, smoking status, body mass index (BMI), systolic and diastolic blood pressure, and moderate-to-vigorous physical activity levels. Multivariate linear regression was used to determine whether CRP was significantly associated with balance function in the US adult population. RESULTS: Simple linear regression showed that CRP was significantly and inversely related to balance function (ß =-0.071, p<0.001). After adjusting for covariates our multivariate linear regression indicated that CRP was significantly and inversely associated with balance function (standardized ß = -0.088, p < 0.001). Other significant contributors to the model included age, education level, SES, BMI (all p < 0.05). CONCLUSION: We found that higher levels of CRP are associated with lower scores on the MRT. These findings suggest that higher systemic inflammation may contribute to increased fall risk in the US adult population. 
   The authors declare no conflicts of interest.

Food insecurity, or limited access to nutritious food, continues to threaten children's physical, emotional, and cognitive development. Our ongoing study explores how these challenges affect families in central Pennsylvania through surveying parents and guardians of children under twelve. Using purposive and convenience sampling, participants are recruited via community partners such as Head Start programs, local food pantries, and family centers. The survey measures diet quality, household stability, parental stress, and overall child well being using validated tools like the USDA HFSSM. Findings, to be presented at the Liberal Arts Symposium, aim to inform community based strategies that address the structural roots of hunger and promote healthier, more equitable childhoods. 

We are looking at food insecurity in our age group and how it can affect physical and mental health outcomes. We hypothesize that food insecurity increases chances for harmful physical and/or mental health outcomes. We are currently conducting our research and will be presenting our findings at LAS. 

Discusses the data and results of Psychophysiological Responses to Primed Intrasexual Competition Vignettes Lab, data collected since 2025. 

Sheetz is working with the I4I team to explore Splunk, a data platform utilized by many companies for logging, analytics, dashboards, etc. The team learned about and utilized Splunk to explore the Sheetz IT environment; With the purpose of exploring potential ways to improve site reliability, create dashboards, and become better prepare the team for the industry, as well as Splunk and products like it. 

Bioproducts in nature can be converted into alternative fuel resources via thermochemical procedures, creating bio-oils. These bio-oils must be separated with solvent extraction through fractionation. Using ultrasound-assisted sequential extraction (UASE) minimizes sample loss, facilitates the concurrent efficient processing of multiple samples, and maximizes solubility for improved fractionation. A past journal has used the UASE approach for lignocellulose pyrolysis fractionation. To study further applications of this relatively novel fractionation process. Synthesis in literature was replicated with dissimilar sample types. Repeated washes with heptane, toluene, and dichloromethane resulted in four fractions to be analyzed separately. Each solvent removed a distinct oil useful for different applications. Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) and high-performance liquid chromatography (HPLC) were utilized in characterizing results. This was in the hopes of confirming the separation of corn stover and pine samples using UASE to be utilized as possible alternatives to fossil fuels.